Protein_Domain

Part:BBa_K2355006

Designed by: Chrysillis Magaard Polhaus   Group: iGEM17_DTU-Denmark   (2017-10-23)


Cleavable peptide sequence from Human Neuromedin-B

This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica and Bitis arietans. The specific peptide sequence of the linker is AAGVAPLS.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Results of experiments

The peptides below, are cleaved by Bitis arietans and Bitis gabonica. It can be used to distinguish between these two Bitis snakes and Naja nigricollis .

In the graf below you are able to see the cleavage by all three venoms.

Substrate screening data for B11'
Figure 1: Substrate with sequence AAGVAPLS (well B11) incubated with the three different venoms.

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Parameters
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